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KMID : 0613820110210010068
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Journal of Life Science
2011 Volume.21 No. 1 p.68 ~ p.73
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Application of β-1,3-Glucanase from Pyrococcus furiosus for Ethanol Production using Laminarin
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Kim Dong-Gyun
Kim Eun-Young
Kim Yu-Ri
Kim Joong-Kyun
Lee Han-Seung
Kong In-Soo
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Abstract
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β-1,3-glucanase from Pyrococcus furiosus was applied for the saccharification of laminarin, which is a major oligo-saccharide component of brown algae, and the reaction mixture produced from laminarin was utilized as a substrate for alcohol fermentation using yeast. To prepare the recombinant β-1,3-glucanase, a β-1,3-glucanase gene was overexpressed in Escherichia coli and purified. Laminarin was degraded to an oligo- and mono-saccharide, such as glucose, after reaction with the purified recombinant β-1,3-glucanase, and the products after enzymatic treatment were confirmed by TLC and HPLC analysis. Decomposed laminarin after enzyme reaction was only added to the medium as a C-source for yeast alcohol production reaction. 0.3% alcohol production was detected from the cultured broth by gas chromatography after 48 hr of incubation. Further evaluation for optimal conditions of saccharification and alcohol fermentation can be suggested, as well as the possibility of using this enzymatic method to produce ethanol using laminarin.
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KEYWORD
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β-1, 3-glucanase, Pyrococcus furiosus, laminarin, ethanol
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